English (United Kingdom)

https://curated-unify.zendy.io/wp-json/zendy-region/v1/featured_content/oa?rat=en

https://curated-unify.zendy.io/wp-json/zendy-region/v1/highlighted_journal/

Global: Zendy Tools annual

Presents the keyphrase highlighting as premium feature

Keyphrase Highlighting

Presents the summarisation as premium feature

Summarisation

Insights

Presents the pdf analysis as premium feature

PDF Analysis

Presents the zaia usage as premium feature

ZAIA

Global: Zendy Tools monthly

Global: Zendy Plus monthly

Presents the access of premium content as premium feature

Premium Content

Global: Zendy Plus annual

Global: Zendy Free Plan

Calreticulin (CRT) is an important molecular chaperon crucial to survival of organisms under adverse conditions. In this study, the potential roles of CRT in the mud crab,  Scylla paramamosain , were investigated. Firstly,  SpCRT  gene expression was detected in various tissues of  S .  paramamosain  with the highest expression found in the hepatopancreas. To evaluate potential role of  SpCRT  in cold adaption, sub-adult crabs were subjected to temperatures of 10, 15, 20 and 25°C and the profiles of  SpCRT  gene were determined in the hepatopancreas, chela muscle and gills. The results showed that the expressions of  SpCRT  mRNA in these tissues were significantly higher for those crabs exposed to low temperatures of 10 and 15°C as compared to those exposed to the higher temperatures, indicating  SpCRT  was involved in cold adaptation—probably through facilitating protein folding. When low temperature 10°C or 15°C was further combined with high and low salinity stress, the expression of  SpCRT  mRNA at low salinity (10 ppt) was in most cases significantly higher than that at high salinity (35 ppt), suggesting that under low temperatures, low salinity may represents a more stressful condition to the crab than high salinity. It was also shown that when crabs challenged by 10°C, Ca 2+  concentration increased rapidly in the hepatopancreas and an  in vitro  experiment further showed that the expression of  SpCRT  mRNA increased concurrently with added Ca 2+  concentration; these results together imply that Ca 2+  probably plays a major role in low temperature signaling, which induces expression of genes related to cold adaption, such as  CRT .

Profiles of calreticulin and Ca2+ concentration under low temperature and salinity stress in the mud crab, Scylla paramamosain