English (United Kingdom)

https://curated-unify.zendy.io/wp-json/zendy-region/v1/featured_content/oa?rat=en

https://curated-unify.zendy.io/wp-json/zendy-region/v1/highlighted_journal/

Global: Zendy Plus annual

Presents the access of premium content as premium feature

Premium Content

Presents the keyphrase highlighting as premium feature

Keyphrase Highlighting

Presents the summarisation as premium feature

Summarisation

Insights

Presents the pdf analysis as premium feature

PDF Analysis

Presents the zaia usage as premium feature

ZAIA

Global: Zendy Tools annual

Global: Zendy Free Plan

Global: Zendy Tools monthly

Global: Zendy Plus monthly

Background   Listeria monocytogenes  is an intracellular foodborne pathogen that employs a number of strategies to survive challenging gastrointestinal conditions. It proliferates in the gut and subsequently causes listeriosis in high-risk individuals. Therefore, inhibition of its adherence to the intestinal receptors is crucial in controlling its infection. In this study, the effect of our previously developed recombinant  Lactobacillus casei  strain expressing invasion protein, Internalin AB of  L .  monocytogenes  (Lbc InlAB ) on epithelial infection processes of the latter under simulated intestinal conditions was investigated.    Materials and methods  The confluent Caco-2 cell monolayer was pre-exposed to different  L .  casei  strains at a multiplicity of exposure (MOE) of 10 for various periods before infection with  L .  monocytogenes  at a multiplicity of infection (MOI) of 10 under simulated intestinal conditions. Subsequently,  L .  monocytogenes  adhesion, invasion, and translocation, cytotoxicity and impact on tight junction integrity of the Caco-2 cells were analyzed.    Results  Under the simulated gastrointestinal condition, Lbc InlAB  showed a significant increase (p<0.0001) in adherence to, invasion and translocation through the Caco-2 cells when compared with the wild type strain. Although Lbc InlAB  strain exhibited enhanced inhibition of  L .  monocytogenes , it was not able to displace  L .  monocytogenes  cells already attached to the monolayer. Additionally, pre-exposure to Lbc InlAB  reduced  L .  monocytogenes -mediated cytotoxicity and protected the tight junction barrier function.    Conclusion  The recombinant  L .  casei  expressing InlAB shows potential for use as a prophylactic intervention strategy for targeted control of  L .  monocytogenes  during the intestinal phase of infection.

plos one

Internalin AB-expressing recombinant Lactobacillus casei protects Caco-2 cells from Listeria monocytogenes-induced damages under simulated intestinal conditions