English (United Kingdom)

https://curated-unify.zendy.io/wp-json/zendy-region/v1/featured_content/oa?rat=en

https://curated-unify.zendy.io/wp-json/zendy-region/v1/highlighted_journal/

Global: Zendy Plus annual

Presents the access of premium content as premium feature

Premium Content

Presents the keyphrase highlighting as premium feature

Keyphrase Highlighting

Presents the summarisation as premium feature

Summarisation

Insights

Presents the pdf analysis as premium feature

PDF Analysis

Presents the zaia usage as premium feature

ZAIA

Global: Zendy Plus monthly

Global: Zendy Tools annual

Global: Zendy Tools monthly

Global: Zendy Free Plan

Erwinia uzenensis  is a plant-pathogenic bacterium, recently described in Japan, which infects pear trees, causing the ‘bacterial black shoot disease of European pear’ (BBSDP). Like other  Erwinia  pear pathogens,  E .  uzenensis  causes damp, black lesions on young shoots resembling those of  E .  amylovora , but not blossom blight, fruitlet blight or wilting of the shoot tip. The distribution of  E .  uzenensis  seems restricted to the country where it was reported up to now, but it may spread to other countries and affect new hosts, as is the current situation with  E .  piriflorinigrans  and  E .  pyrifoliae . Fast and accurate detection systems for this new pathogen are needed to study its biology and to identify it on pear or other hosts. We report here the development of a specific and sensitive detection protocol based on a real-time PCR with a TaqMan probe for  E .  uzenensis , and its evaluation. In sensitivity assays, the detection threshold of this protocol was 10 1  cfu ml -1  on pure bacterial cultures and 10 2 –10 3  cfu ml -1  on spiked plant material. The specificity of the protocol was evaluated against  E .  uzenensis  and 46 strains of pear-associated  Erwinia  species different to  E .  uzenensis . No cross-reaction with the non-target bacterial species or the loss of sensitivity were observed. This specific and sensitive diagnostic tool may reveal a wider distribution and host range of  E .  uzenensis  initially considered restricted to a region and will expand our knowledge of the life cycle and environmental preferences of this pathogen.

Development of a real-time PCR method for the specific detection of the novel pear pathogen Erwinia uzenensis