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Multidrug resistance efflux pumps protect bacterial cells against a wide spectrum of antimicrobial compounds. PSPTO_0820 is a predicted multidrug transporter from the phytopathogenic bacterium  Pseudomonas syringae  pv. tomato DC3000. Orthologs of this protein are conserved within many  Pseudomonas  species that interact with plants. To study the potential role of  PSPTO_0820  in plant-bacteria interaction, a mutant in this gene was isolated and characterized. In addition, with the aim to find the outer membrane channel for this efflux system, a mutant in  PSPTO_4977 , a TolC-like gene, was also analyzed. Both mutants were more susceptible to  trans -cinnamic and chlorogenic acids and to the flavonoid (+)-catechin, when added to the culture medium. The expression level of both genes increased in the presence of (+)-catechin and, in the case of  PSPTO_0820 , also in response to  trans -cinnamic acid. PSPTO_0820 and PSPTO_4977 mutants were unable to colonize tomato at high population levels. This work evidences the involvement of these two proteins in the resistance to plant antimicrobials, supporting also the importance of chlorogenic acid,  trans -cinnamic acid, and (+)-catechin in the tomato plant defense response against  P .  syringae  pv. tomato DC3000 infection.

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The Pseudomonas syringae pv. tomato DC3000 PSPTO_0820 multidrug transporter is involved in resistance to plant antimicrobials and bacterial survival during tomato plant infection