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Polycomb group (PcG) proteins are essential regulators of epigenetic gene silencing and development. The PcG protein enhancer of zeste homolog 2 (Ezh2) is a key component of the Polycomb Repressive Complex 2 and is responsible for placing the histone H3 lysine 27 trimethylation (H3K27me3) repressive mark on the genome through its methyltransferase domain. Ezh2 is highly conserved in vertebrates. We studied the role of  ezh2  during development of zebrafish with the use of a mutant allele ( ezh2(sa1199) , R18STOP), which has a stop mutation in the second exon of the  ezh2  gene. Two versions of the same line were used during this study. The first and original version of zygotic  ezh2(sa1199)  mutants unexpectedly retained  ezh2  expression in brain, gut, branchial arches, and eyes at 3 days post-fertilization (dpf), as revealed by in-situ hybridization. Moreover, the expression pattern in homozygous mutants was identical to that of wild types, indicating that mutant  ezh2  mRNA is not subject to nonsense mediated decay (NMD) as predicted. Both wild type and  ezh2  mutant embryos presented edemas at 2 and 3 dpf. The line was renewed by selective breeding to counter select the non-specific phenotypes and survival was assessed. In contrast to earlier studies on  ezh2  mutant zebrafish,  ezh2(sa1199)  mutants survived until adulthood. Interestingly, the  ezh2  mRNA and Ezh2 protein were present during adulthood (70 dpf) in both wild type and  ezh2(sa1199)  mutant zebrafish. We conclude that the  ezh2(sa1199)  allele does not exhibit an  ezh2  loss-of-function phenotype.

The ezh2(sa1199) mutant zebrafish display no distinct phenotype