English (United Kingdom)

https://curated-unify.zendy.io/wp-json/zendy-region/v1/featured_content/oa?rat=en

https://curated-unify.zendy.io/wp-json/zendy-region/v1/highlighted_journal/

Global: Zendy Plus annual

Presents the access of premium content as premium feature

Premium Content

Presents the keyphrase highlighting as premium feature

Keyphrase Highlighting

Presents the summarisation as premium feature

Summarisation

Insights

Presents the pdf analysis as premium feature

PDF Analysis

Presents the zaia usage as premium feature

ZAIA

Global: Zendy Tools annual

Global: Zendy Free Plan

Global: Zendy Tools monthly

Global: Zendy Plus monthly

Identifying mosquito species is a fundamental step in risk assessment and implementation of preventative strategies. Moreover,  Culex pipiens  is the most widespread mosquito vector in several regions of Iran and is the main vector for transmission of West Nile virus (WNV). Mosquitoes were collected at 14 sites in northern regions of Iran in 2015 and 2016. A subset of mosquito specimens was selected for identification confirmation using a DNA-barcoding technique. Construction of a phylogenetic tree showed clustering of mosquito sequences into three main genera:  Aedes ,  Anopheles  and  Culex  with individuals of a single species clustered closely together, regardless of where and when they were collected.  Cx .  pipiens  complex and  Cx .  torrentium  were identified and differentiated using multiplex real-time PCR targeting the gene locus for acetylcholinesterase 2 (ace2) to discriminate between  Cx .  pipiens pipiens  and  Cx .  torrentium . The CQ11 microsatellite locus was used for discrimination between  Cpp . biotypes. The predominant mosquito species in investigated regions were  Cx .  pipiens pipiens  biotype  pipiens , but we also detected  Culex pipiens pipiens  biotype  molestus  and hybrids of the two  pipiens  biotypes, as well as  Cx .  torrentium . The results of this study represent the first certain evidence of the presence of  Cx .  pipiens pipiens  biotype  molestus  and hybrids between  pipiens  and  molestus  forms, and  Cx .  torrentium  in Iran through a molecular identification approach. This report of a potentially important bridge vector for WNV might have key influence in the risk projections for WNV in Iran.

plos one

DNA barcodes corroborating identification of mosquito species and multiplex real-time PCR differentiating Culex pipiens complex and Culex torrentium in Iran