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Heat shock proteins (HSPs) are a type of conserved molecular chaperone. They exist extensively in plants and greatly contribute to their survival under heat stress. The transcriptional regulation factor heat shock factor (HSF) is thought to regulate the expression of  Hsps . In this study, a novel gene designated  BcHsfA1  was cloned and characterized from  Brassica campestris . Bioinformatic analysis implied that  BcHsfA1  belongs to the  HsfA  gene family and is most closely related to  HsfA1  from other plants. Constitutive overexpression of  BcHsfA1  significantly improved heat tolerance of tobacco seedlings by affecting physiological and biochemical processes. Moreover, the chlorophyll content of transgenic tobacco plants was significantly increased compared with wild type after heat stress, as were the activities of the important enzymatic antioxidants superoxide dismutase and peroxidase.  BcHsfA1  overexpression also resulted in decreased malondialdehyde content and comparative electrical conductivity and increased soluble sugar content in transgenic tobacco plants than wild-type plants exposed to heat stress. Furthermore, we identified 11 candidate heat response genes that were significantly up-regulated in the transgenic lines exposed to heat stress. Together, these results suggested that  BcHsfA1  is effective in improving heat tolerance of tobacco seedlings, which may be useful in the development of new heat-resisitant  B .  campestris  strains by genetic engineering.

Overexpression of BcHsfA1 transcription factor from Brassica campestris improved heat tolerance of transgenic tobacco