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Objective  The objective of the research was to assess the susceptibility of the slowly growing nontuberculous mycobacteria strains to the antimicrobial drugs used for mycobaterioses treatment using SLOMYCO test system.    Materials and methods  We assessed 363 NTM strains: 177 MAC (161  M .  avium , 16  M .  intracellulare ), 112  M .  kansasii  and 74  M .  xenopi  collected from the respiratory material of the patients were under the treatment or under diagnostic procedures at our Center, affiliates and the diagnostic department in 2010–2016. Drug sucseptibility for NTM was tested using the Sensititre SLOWMYCO system (TREK DIAGNOSTIC Systems Ltd., UK). MICs were established by microdilutions in Mueller-Hinton broth on polystyrene 96-well plates. The statistical analysis was done using the StatGraphics Plus 5.0 software. The data were compared pairwise using Pearson χ 2  test with Yates correction. 95% confidence interval (CI) were calculated. Statistically significant differences were considered for p <0.05. Log-rank test and Kaplan-Meier curves were used to assess the concentration-dependent surveillance probability.    Results  The statistically significant differences were revealed in sensitivity/resistance isolates of  M .  avium  and  M .  intracellulare :  M .  avium  strains were resistant to higher concentrations of amikacin, clarithromycin, linezolid and streptomycin (p <0.01);  M .  intracellulare  strains were resistant to higher concentrations of ethionamide (p <0.05). The isolates of  M .  avium  were significantly more resistant than  M .  kansasii  to amikacin, doxycycline, isoniazid, clarithromycin, linezolid, moxifloxacin, rifabutin, rifampicin, streptomycin, trimethoprim/sulfamethoxazole, ciprofloxacin, ethambutol, ethionamide (visible growth of  M .  avium  were inhibited by higher drug concentrations, p <0.01). The isolates of  M .  avium  showed significantly higher resistance than  M .  xenopi  to amikacin, doxycycline, isoniazid, clarithromycin, linezolid, moxifloxacin, rifampicin, streptomycin, trimethoprim/sulfamethoxazole, ciprofloxacin, ethambutol, and ethionamide (visible growth of  M .  avium  were inhibited by higher drug concentrations, p <0.01). Statistically significant differences in the dynamics of the response to the antibacterial effects of isoniazid, linezolid, moxifloxacin, rifampicin, trimethoprim/sulfamethoxazole, ethambutol, and ethionamide were found for  M .  intracellulare  and  M .  xenopi  (complete inhibition of the visible growth of  M .  intracellulare  required higher drugs concentrations, p <0, 05). Comparison of the Kaplan-Meyer curves revealed statistically significant differences in survialence probability of  M .  kansasii  and  M .  xenopi  for amikacin, doxycycline, rifampicin, trimethoprim/sulfamethoxazole, ciprofloxacin, ethambutol, and ethionamide (a higher number of isolates of  M .  xenopi  were inhibited by low drugs concentrations, p <0.05).    Conclusions  Our data show that  M .  avium  and  M .  intracellulare  were more resistant to the majority of the studied drugs than  M .  kansasii  and  M .  xenopi .

Drug susceptibility testing of slowly growing non-tuberculous mycobacteria using slomyco test-system