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Seed exudates are plant-derived natural bioactive compounds consisting of a complex mixture of organic and inorganic molecules. Plant seed exudates have been poorly studied against parasite nematodes. This study was undertaken to identify proteins in the  Myracrodruon urundeuva  seed exudates and to assess the anthelmintic activity against  Haemonchus contortus , an important parasite of small ruminants.  M .  urundeuva  seed exudates (SEX) was obtained after immersion of seeds in sodium acetate buffer. SEX was fractionated with ammonium sulfate at 0–90% concentration to generate the ressuspended pellet (SEXF1) and the supernatant (SEXF2). SEX, SEXF1, and SEXF2 were exhaustively dialyzed against distilled water (cut-off: 12 kDa) and the protein contents determined. Mass spectrometry analyses of SEX, SEXF1, and SEXF2 were done to identify proteins and secondary metabolites. The seed exudates contained protease, protease inhibitor, peptidase, chitinase, and lipases as well as the low molecular weight secondary compounds ellagic acid and quercetin rhamnoside. SEX inhibited  H .  contortus  larval development (LDA) (IC 50  = 0.29 mg mL -1 ), but did not affect larval exsheathment (LEIA). On the other hand, although SEXF1 and SEXF2 inhibited  H .  contortus  LEIA (IC 50  = 1.04 and 0.93 mg mL -1 , respectively), they showed even greater inhibition efficiency of  H .  contortus  larval development (IC 50  = 0.29 and 0.42 mg mL -1 , respectively). To the best of our knowledge, this study is the first to show the anthelmintic activity of plant exudates against a gastrointestinal nematode. Moreover, it suggests the potential of exuded proteins as candidates to negatively interfere with  H .  contortus  life cycle.

Myracrodruon urundeuva seed exudates proteome and anthelmintic activity against Haemonchus contortus