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Background  To determine the validity of the lactate threshold (LT) and maximal oxygen uptake (V  ˙   O    2  max) determined during graded exercise test (GXT) of different durations and using different LT calculations. Trained male cyclists (n = 17) completed five GXTs of varying stage length (1, 3, 4, 7 and 10 min) to establish the LT, and a series of 30-min constant power bouts to establish the maximal lactate steady state (MLSS).V  ˙   O    2was assessed during each GXT and a subsequent verification exhaustive bout (VEB), and 14 different LTs were calculated from four of the GXTs (3, 4, 7 and 10 min)—yielding a total 56 LTs. Agreement was assessed between the highestV  ˙   O    2measured during each GXT (V  ˙   O    2  peak) as well as between each LT and MLSS.V  ˙   O    2  peakand LT data were analysed using mean difference (MD) and intraclass correlation (ICC).    Results  TheV  ˙   O    2  peakvalue from GXT 1  was 61.0 ± 5.3 mL . kg -1. min -1  and the peak power 420 ± 55 W (mean ± SD). The power at the MLSS was 264 ± 39 W.V  ˙   O    2  peakfrom GXT 3, 4, 7, 10  underestimatedV  ˙   O    2  peakby ~1–5 mL . kg -1. min -1 . Many of the traditional LT methods were not valid and a newly developed Modified D max  method derived from GXT 4  provided the most valid estimate of the MLSS (MD = 1.1 W; ICC = 0.96).    Conclusion  The data highlight how GXT protocol design and data analysis influence the determination of bothV  ˙   O    2  peakand LT. It is also apparent thatV  ˙   O    2  maxand LT cannot be determined in a single GXT, even with the inclusion of a VEB.

Manipulating graded exercise test variables affects the validity of the lactate threshold and V˙O2peak