English (United Kingdom)

https://curated-unify.zendy.io/wp-json/zendy-region/v1/featured_content/oa?rat=en

https://curated-unify.zendy.io/wp-json/zendy-region/v1/highlighted_journal/

Global: Zendy Plus annual

Presents the access of premium content as premium feature

Premium Content

Presents the keyphrase highlighting as premium feature

Keyphrase Highlighting

Presents the summarisation as premium feature

Summarisation

Insights

Presents the pdf analysis as premium feature

PDF Analysis

Presents the zaia usage as premium feature

ZAIA

Global: Zendy Plus monthly

Global: Zendy Tools annual

Global: Zendy Tools monthly

Global: Zendy Free Plan

Background  Genetic risk factors, intestinal microbiota and a dysregulated immune system contribute to the pathogenesis of inflammatory bowel disease (IBD). We have previously demonstrated that dysfunction of protein tyrosine phosphatase non-receptor type 2 (PTPN2) and PTPN22 contributes to alterations of intestinal microbiota and the onset of chronic intestinal inflammation  in vivo . Here, we investigated the influence of PTPN2 and PTPN22 gene variants on intestinal microbiota composition in IBD patients.    Methods  Bacterial DNA from mucosa-associated samples of 75 CD and 57 UC patients were sequenced using 16S rRNA sequencing approach. Microbial analysis, including alpha diversity, beta diversity and taxonomical analysis by comparing to PTPN2 (rs1893217) and PTPN22 (rs2476601) genotypes was performed in QIIME, the  phyloseq  R package and MaAsLin pipeline.    Results  In PTPN2 variant UC patients, we detected an increase in relative abundance of unassigned genera from  Clostridiales  and  Lachnospiraceae  families and reduction of  Roseburia  when compared to PTPN2 wild-type (WT) patients.  Ruminoccocus  was increased in PTPN22 variant UC patients. In CD patients with severe disease course,  Faecalibacterium ,  Bilophila ,  Coprococcus , unclassified  Erysipelotrichaeceae , unassigned genera from  Clostridiales  and  Ruminococcaceae  families were reduced and  Bacteroides  were increased in PTPN2 WT carriers, while  Faecalibacterium ,  Bilophila ,  Coprococcus , and  Erysipelotrichaeceae  were reduced in PTPN22 WT patients when compared to patients with mild disease. In UC patients with severe disease, relative abundance of  Lachnobacterium  was reduced in PTPN2 and PTPN22 WT patients,  Dorea  was increased in samples from PTPN22 WT carriers and an unassigned genus from  Ruminococcaceae gen . was increased in patients with PTPN2 variant genotype.    Conclusions  We identified that IBD-associated genetic risk variants, disease severity and the interaction of these factors are related to significant alterations in intestinal microbiota composition of IBD patients.

The presence of genetic risk variants within PTPN2 and PTPN22 is associated with intestinal microbiota alterations in Swiss IBD cohort patients