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Pseudomonas syringae  Lz4W RecBCD enzyme, RecBCD Ps , is a trimeric protein complex comprised of RecC, RecB, and RecD subunits. RecBCD enzyme is essential for  P .  syringae  growth at low temperature, and it protects cells from low temperature induced replication arrest. In this study, we show that the RecBCD Ps  enzyme displays distinct biochemical behaviors. Unlike  E .  coli  RecBCD enzyme, the RecD subunit is indispensable for RecBCD Ps  function. The RecD motor activity is essential for the Chi-like fragments production in  P .  syringae , highlighting a distinct role for  P .  syringae  RecD subunit in DNA repair and recombination process. Here, we demonstrate that the RecBCD Ps  enzyme recognizes a unique octameric DNA sequence,  5′-GCTGGCGC-3′  (Chi Ps ) that attenuates nuclease activity of the enzyme when it enters dsDNA from the 3′-end. We propose that the reduced translocation activities manifested by motor-defective mutants cause cold sensitivity in  P .  syrinage ; emphasizing the importance of DNA processing and recombination functions in rescuing low temperature induced replication fork arrest.

Biochemical characterization of RecBCD enzyme from an Antarctic Pseudomonas species and identification of its cognate Chi (χ) sequence