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Context  The influence of methodological parameters on the measurement of muscle contractile properties using Tensiomyography (TMG) has not been published.    Objective  To investigate the; (1) reliability of stimulus amplitude needed to elicit maximum muscle displacement (Dm), (2) effect of changing inter-stimulus interval on Dm (using a fixed stimulus amplitude) and contraction time (T c ), (3) the effect of changing inter-electrode distance on Dm and T c .    Design  Within subject, repeated measures.    Participants  10 participants for each objective.    Main outcome measures  Dm and T c  of the rectus femoris, measured using TMG.    Results  The coefficient of variance (CV) and the intra-class correlation (ICC) of stimulus amplitude needed to elicit maximum Dm was 5.7% and 0.92 respectively. Dm was higher when using an inter-electrode distance of 7cm compared to 5cm [ P  = 0.03] and when using an inter-stimulus interval of 10s compared to 30s [ P  = 0.017]. Further analysis of inter-stimulus interval data, found that during 10 repeated stimuli T c  became faster after the 5 th  measure when compared to the second measure [P<0.05]. The 30s inter-stimulus interval produced the most stable T c  over 10 measures compared to 10s and 5s respectively.    Conclusion  Our data suggest that the stimulus amplitude producing maximum Dm of the rectus femoris is reliable. Inter-electrode distance and inter-stimulus interval can significantly influence Dm and/ or T c . Our results support the use of a 30s inter-stimulus interval over 10s or 5s. Future studies should determine the influence of methodological parameters on muscle contractile properties in a range of muscles.

Repeated stimulation, inter-stimulus interval and inter-electrode distance alters muscle contractile properties as measured by Tensiomyography