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Objective  To investigate the effects of four strains, generally used in clinic, including  Bifidobacterium longum ,  Lactobacillus acidophilus ,  Streptococcus thermophilus  and  Enterococcus faecalis , and their related products on human colonic smooth muscle  in vitro .    Methods  Human colonic circular muscle strips obtained from disease-free margins of resected segments from 25 patients with colorectal cancer were isometrically examined in a constant-temperature organ bath and exposed to different concentrations of living bacteria, sonicated cell fractions and cell-free supernatant (CFS). The area under the curve (AUC) representing the contractility of smooth muscle strips was calculated.    Results  (1) The four living probiotics inhibited the contractility of human colonic muscle strips only at high concentration (10 10  CFUs/mL, all  P <0.05). (2) The sonicated cell fractions from the four probiotics obviously inhibited human colonic smooth muscle strips in a dose-dependent manner ( P <0.01). (3) The CFS from the four probiotics also inhibited colonic smooth muscle strips in a dose-dependent manner (all  P <0.05). (4) The inhibition effect of CFS from  Streptococcus thermophilus  and  Enterococcus faecalis  decreased obviously when pretreated with NG-nitro-L-arginine (L-NNA, 10 −5  mol/L) ( P <0.05), but not the  Bifidobacterium longum  and  Lactobacillus acidophilus  ( P >0.05).    Conclusion  Four common probiotics related products, including the sonicated cell fractions and the CFS, obviously inhibited human colonic smooth muscles strips contraction in a dose-dependent manner. Only high concentration living probiotics (10 10  CFUs/mL) can inhibit the colonic smooth muscles strips contraction. The NO pathway may be partly involved in the inhibitory effect of CFS from  Streptococcus thermophilus  and  Enterococcus faecalis .

plos one

Inhibition effect of Bifidobacterium longum, Lactobacillus acidophilus, Streptococcus thermophilus and Enterococcus faecalis and their related products on human colonic smooth muscle in vitro