English (United Kingdom)

https://curated-unify.zendy.io/wp-json/zendy-region/v1/featured_content/oa?rat=en

https://curated-unify.zendy.io/wp-json/zendy-region/v1/highlighted_journal/

Global: Zendy Plus annual

Presents the access of premium content as premium feature

Premium Content

Presents the keyphrase highlighting as premium feature

Keyphrase Highlighting

Presents the summarisation as premium feature

Summarisation

Insights

Presents the pdf analysis as premium feature

PDF Analysis

Presents the zaia usage as premium feature

ZAIA

Global: Zendy Tools annual

Global: Zendy Free Plan

Global: Zendy Tools monthly

Global: Zendy Plus monthly

Trichomonas vaginalis  and  Tritrichomonas foetus  are pathogens that parasitise, respectively, human and bovine urogenital tracts causing disease. Using LC-MS, reference metabolomic profiles were obtained for both species and stable isotope labelling with D-[U- 13 C6] glucose was used to analyse central carbon metabolism. This facilitated a comparison of the metabolic pathways of  T .  vaginalis  and  T .  foetus , extending earlier targeted biochemical studies. 43 metabolites, whose identities were confirmed by comparison of their retention times with authentic standards, occurred at more than 3-fold difference in peak intensity between  T .  vaginalis  and  T .  foetus . 18 metabolites that were removed from or released into the medium during growth also showed more than 3-fold difference between the species. Major differences were observed in cysteine and methionine metabolism in which homocysteine, produced as a bi-product of trans-methylation, is catabolised by methionine γ-lyase in  T .  vaginalis  but converted to cystathionine in  T .  foetus . Both species synthesise methylthioadenosine by an unusual mechanism, but it is not used as a substrate for methionine recycling.  T .  vaginalis  also produces and exports high levels of S-methylcysteine, whereas only negligible levels were found in  T .  foetus  which maintains significantly higher intracellular levels of cysteine.  13 C-labeling confirmed that both cysteine and S-methylcysteine are synthesised by  T .  vaginalis ; S-methylcysteine can be generated by recombinant  T .  vaginalis  cysteine synthase using phosphoserine and methanethiol.  T .  foetus  contained higher levels of ornithine and citrulline than  T .  vaginalis  and exported increased levels of putrescine, suggesting greater flux through the arginine dihydrolase pathway.  T .  vaginalis  produced and exported hydroxy acid derivatives of certain amino acids, particularly 2-hydroxyisocaproic acid derived from leucine, whereas negligible levels of these metabolites occurred in  T .  foetus .

plos one

Metabolomic profiling and stable isotope labelling of Trichomonas vaginalis and Tritrichomonas foetus reveal major differences in amino acid metabolism including the production of 2-hydroxyisocaproic acid, cystathionine and S-methylcysteine