English (United Kingdom)

https://curated-unify.zendy.io/wp-json/zendy-region/v1/featured_content/oa?rat=en

https://curated-unify.zendy.io/wp-json/zendy-region/v1/highlighted_journal/

Global: Zendy Plus annual

Presents the access of premium content as premium feature

Premium Content

Presents the keyphrase highlighting as premium feature

Keyphrase Highlighting

Presents the summarisation as premium feature

Summarisation

Insights

Presents the pdf analysis as premium feature

PDF Analysis

Presents the zaia usage as premium feature

ZAIA

Global: Zendy Tools annual

Global: Zendy Free Plan

Global: Zendy Tools monthly

Global: Zendy Plus monthly

Telomeres, the nucleoprotein complexes at the termini of linear chromosomes,are essential for the processes of end replication, end-protection, andchromatin segregation. The Mre11 complex is involved in multiple cellular rolesin DNA repair and structure in the regulation and function of telomere sizehomeostasis. In this study, we characterize yeast telomere chromatin structure,phenotypic heritability, and chromatin segregation in both wild-type [MRE11]and A470 motif alleles. MRE11 strains confer a telomere size of 300 base pairsof G+T irregular simple sequence repeats. This DNA and a portion ofsubtelomeric DNA is embedded in a telosome: an MNase-resistant non-nucleosomalparticle. Chromatin immunoprecipitation shows a three to four-fold loweroccupancy of Mre11A470T proteins than wild-type proteins in telosomes.Telosomes containing the Mre11A470T protein confer a greater resistance toMNase digestion than wild-type telosomes. The integration of a wild-type MRE11allele into an ectopic locus in the genome of a mre11A470T mutant and theintroduction of a mre11A470T allele at an ectopic site in a wild-type strainlead to unexpectedly differing results. In each case, the replicated sisterchromatids inherit telosomes containing only the protein encoded by the genomicmre11 locus, even in the presence of protein encoded by the opposing ectopicallele. We hypothesize that the telosome segregates by a conservativemechanism. These data support a mechanism for the linkage between sisterchromatid replication and maintenance of either identical mutant or identicalwild-type telosomes after replication of sister chromatids. These data suggestthe presence of an active mechanism for chromatin segregation in yeast.

The mre11 A470 Alleles Influence the. Heritability and Segregation of Telosomes in Saccharomyces cerevisiae