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Fast, label-free, high-resolution, three-dimensional imaging platforms are crucial for high-throughput  in vivo  time-lapse studies of the anatomy of  Caenorhabditis elegans , one of the most commonly used model organisms in biomedical research. Despite the needs, methods combining all these characteristics have been lacking. Here, we present label-free imaging of live  Caenorhabditis elegans  with three-dimensional sub-micrometer resolution using visible optical coherence microscopy (visOCM). visOCM is a versatile optical imaging method which we introduced recently for tomography of cell cultures and tissue samples. Our method is based on Fourier domain optical coherence tomography, an interferometric technique that provides three-dimensional images with high sensitivity, high acquisition rate and micrometer-scale resolution. By operating in the visible wavelength range and using a high NA objective, visOCM attains lateral and axial resolutions below 1 μm. Additionally, we use a Bessel illumination offering an extended depth of field of approximately 40 μm. We demonstrate that visOCM’s imaging properties allow rapid imaging of full sized living  Caenorhabditis elegans  down to the sub-cellular level. Our system opens the door to many applications such as the study of phenotypic changes related to developmental or ageing processes.

Label-free three-dimensional imaging of Caenorhabditis elegans with visible optical coherence microscopy