English (United Kingdom)

https://curated-unify.zendy.io/wp-json/zendy-region/v1/featured_content/oa?rat=en

https://curated-unify.zendy.io/wp-json/zendy-region/v1/highlighted_journal/

Global: Zendy Plus annual

Presents the access of premium content as premium feature

Premium Content

Presents the keyphrase highlighting as premium feature

Keyphrase Highlighting

Presents the summarisation as premium feature

Summarisation

Insights

Presents the pdf analysis as premium feature

PDF Analysis

Presents the zaia usage as premium feature

ZAIA

Global: Zendy Tools annual

Global: Zendy Free Plan

Global: Zendy Tools monthly

Global: Zendy Plus monthly

The promoter selectivity of  Escherichia coli  RNA polymerase (RNAP) is determined by the sigma subunit. The model prokaryote  Escherichia coli  K-12 contains seven species of the sigma subunit, each recognizing a specific set of promoters. For identification of the “constitutive promoters” that are recognized by each RNAP holoenzyme alone in the absence of other supporting factors, we have performed the genomic SELEX screening  in vitro  for their binding sites along the  E .  coli  K-12 W3110 genome using each of the reconstituted RNAP holoenzymes and a collection of genome DNA segments of  E .  coli  K-12. The whole set of constitutive promoters for each RNAP holoenzyme was then estimated based on the location of RNAP-binding sites. The first successful screening of the constitutive promoters was achieved for RpoD (σ 70 ), the principal sigma for transcription of growth-related genes. As an extension, we performed in this study the screening of constitutive promoters for four minor sigma subunits, stationary-phase specific RpoS (σ 38 ), heat-shock specific RpoH (σ 32 ), flagellar-chemotaxis specific RpoF (σ 28 ) and extra-cytoplasmic stress-response RpoE (σ 24 ). The total number of constitutive promoters were: 129~179 for RpoS; 101~142 for RpoH; 34~41 for RpoF; and 77~106 for RpoE. The list of constitutive promoters were compared with that of known promoters identified  in vivo  under various conditions and using varieties of  E .  coli  strains, altogether allowing the estimation of “inducible promoters” in the presence of additional supporting factors.

The whole set of the constitutive promoters recognized by four minor sigma subunits of Escherichia coli RNA polymerase