English (United Kingdom)

https://curated-unify.zendy.io/wp-json/zendy-region/v1/featured_content/oa?rat=en

https://curated-unify.zendy.io/wp-json/zendy-region/v1/highlighted_journal/

Global: Zendy Plus annual

Presents the access of premium content as premium feature

Premium Content

Presents the keyphrase highlighting as premium feature

Keyphrase Highlighting

Presents the summarisation as premium feature

Summarisation

Insights

Presents the pdf analysis as premium feature

PDF Analysis

Presents the zaia usage as premium feature

ZAIA

Global: Zendy Tools annual

Global: Zendy Free Plan

Global: Zendy Tools monthly

Global: Zendy Plus monthly

In this study, GelGreen ™  was investigated as a replacement for SYBR ®  Safe to stain DNA in cesium chloride (CsCl) density gradients for DNA-stable isotope probing (SIP) experiments. Using environmental DNA, the usage of GelGreen ™  was optimized for sensitivity compared to SYBR ®  Safe, its optimal concentration, detection limit for environmental DNA and its application in environmental DNA-SIP assay. Results showed that GelGreen ™  was more sensitive than SYBR ®  Safe, while the optimal dosage (15X concentration) needed was approximately one-third of SYBR ®  Safe, suggesting that its sensitivity was three times more superior than SYBR ®  Safe. At these optimal parameters, the detection limit of GelGreen ™ -stained environmental DNA was as low as 0.2 μg, but the usage of 0.5 μg environmental DNA was recommended to produce a more consistent DNA band. In addition, a modified needle extraction procedure was developed to withdraw DNA effectively by fractionating CsCl density gradients into four or five fractions. The successful application of GelGreen ™  staining with  13 C-labeled DNA from enriched activated sludge suggests that this stain was an excellent alternative of SYBR ®  Safe in CsCl density gradients for DNA-SIP assays.

Application of GelGreen™ in Cesium Chloride Density Gradients for DNA-Stable Isotope Probing Experiments