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Biofilms are a key component in bacterial communities providing protection and contributing to infectious diseases. However, mechanisms involved in  S .  sanguinis  biofilm formation have not been clearly elucidated. Here, we report the identification of a novel  S .  sanguinis  TetR repressor,  brpT  ( B iofilm  R egulatory  P rotein  T etR), involved in biofilm formation. Deletion of  brpT  resulted in a significant increase in biofilm formation. Interestingly, the mutant accumulated more water soluble and water insoluble glucans in its biofilm compared to the wild-type and the complemented mutant. The  brpT  mutation led to an altered biofilm morphology and structure exhibiting a rougher appearance, uneven distribution with more filaments bound to the chains. RNA-sequencing revealed that  gtfP , the only glucosyltransferase present in  S .  sanguinis , was significantly up-regulated. In agreement with these findings, we independently observed that deletion of  gtfP  in  S .  sanguinis  led to reduced biofilm and low levels of water soluble and insoluble glucans. These results suggest that  brpT  is involved in the regulation of the  gtfP -mediated exopolysaccharide synthesis and controls  S .  sanguinis  biofilm formation. The deletion of  brpT  may have a potential therapeutic application in regulating  S .  sanguinis  colonization in the oral cavity and the prevention of dental caries.

TetR Family Regulator brpT Modulates Biofilm Formation in Streptococcus sanguinis