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Chronic non-progressive pneumonia, a disease that has become a worldwide epidemic has caused considerable loss to sheep industry.  Mycoplasma ovipneumoniae  ( M .  ovipneumoniae ) is the causative agent of interstitial pneumonia in sheep, goat and bighorn. We here have identified by immunogold and immunoblotting that elongation factor Tu (EF-Tu) and heat shock protein 70 (HSP 70) are membrane-associated proteins on  M .  ovipneumonaiea . We have evaluated the humoral and cellular immune responses  in vivo  by immunizing BALB/c mice with both purified recombinant proteins rEF-Tu and rHSP70. The sera of both rEF-Tu and rHSP70 treated BALB/c mice demonstrated increased levels of IgG, IFN-γ, TNF-α, IL-12(p70), IL-4, IL-5 and IL-6. In addition, ELISPOT assay showed significant increase in IFN-γ +  secreting lymphocytes in the rHSP70 group when compared to other groups. Collectively our study reveals that rHSP70 induces a significantly better cellular immune response in mice, and may act as a Th1 cytokine-like adjuvant in immune response induction. Finally, growth inhibition test (GIT) of  M .  ovipneumoniae  strain Y98 showed that sera from rHSP70 or rEF-Tu-immunized mice inhibited  in vitro  growth of  M .  ovipneumoniae . Our data strongly suggest that EF-Tu and HSP70 of  M .  ovipneumoniae  are membrane-associated proteins capable of inducing antibody production, and cytokine secretion. Therefore, these two proteins may be potential candidates for vaccine development against  M .  ovipneumoniae  infection in sheep.

plos one

Elongation Factor Tu and Heat Shock Protein 70 Are Membrane-Associated Proteins from Mycoplasma ovipneumoniae Capable of Inducing Strong Immune Response in Mice