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Angiostrongylus cantonensis  is a zoonotic nematode parasite causing human eosinophilic meningitis (or meningoencephalitis) worldwide. A closely related species,  Angiostrongylus malaysiensis , might also be a human pathogen. Larvae were obtained from land snails in Lao PDR, Cambodia, Myanmar and Thailand. We sequenced two nuclear gene regions (nuclear ribosomal ITS2 and SSU rRNA) and a portion of one mitochondrial gene (COI) from these larvae.  Angiostrongylus cantonensis  and  A .  malaysiensis  were identified. This is the first report of the molecular identification of the two  Angiostrongylus  species in Lao PDR, Cambodia and Myanmar. The regional distributions of the two species broadly overlap. Phylogenetic relationships were inferred including data from  Angiostrongylus  species deposited in public databases. All the gene regions we sequenced have potential value in distinguishing between species of  Angiostrongylus . The COI gene exhibited the greatest intraspecific variation in the study region (five haplotypes in  A .  cantonensis  and four in  A .  malaysiensis ) and might be suitable for more detailed phylogeographic studies.

plos one

Angiostrongylus cantonensis and A. malaysiensis Broadly Overlap in Thailand, Lao PDR, Cambodia and Myanmar: A Molecular Survey of Larvae in Land Snails