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The genetic lineages of  Listeria monocytogenes  and other species of the genus  Listeria  are correlated with pathogenesis in humans. Although matrix-assisted laser desorption ionization-time of flight mass spectrometry (MALDI-TOF MS) has become a prevailing tool for rapid and reliable microbial identification, the precise discrimination of  Listeria  species and lineages remains a crucial issue in clinical settings and for food safety. In this study, we constructed an accurate and reliable MS database to discriminate the lineages of  L .  monocytogenes  and the species of  Listeria  ( L .  monocytogenes ,  L .  innocua ,  L .  welshimeri ,  L .  seeligeri ,  L .  ivanovii ,  L .  grayi , and  L .  rocourtiae ) based on the  S10-spc-alpha  operon gene encoded ribosomal protein mass spectrum ( S10 -GERMS) proteotyping method, which relies on both genetic information (genomics) and observed MS peaks in MALDI-TOF MS (proteomics). The specific set of eight biomarkers (ribosomal proteins L24, L6, L18, L15, S11, S9, L31 type B, and S16) yielded characteristic MS patterns for the lineages of  L .  monocytogenes  and the different species of  Listeria , and led to the construction of a MS database that was successful in discriminating between these organisms in MALDI-TOF MS fingerprinting analysis followed by advanced proteotyping software Strain Solution analysis. We also confirmed the constructed database on the proteotyping software Strain Solution by using 23  Listeria  strains collected from natural sources.

plos one

Matrix-assisted Laser Desorption Ionization-Time of Flight Mass Spectrometry (MALDI-TOF MS) Can Precisely Discriminate the Lineages of Listeria monocytogenes and Species of Listeria