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The aim of current study was to examine clonal structure and genetic profile of invasive  Staphylococcus aureus  isolates recovered from infants and children treated at the Jagiellonian University Children’s Hospital of Krakow, Poland. The 107 invasive  S .  aureus  isolates, collected between February 2012 and August 2014, were analysed retrospectively. Antimicrobial susceptibility testing,  spa  typing and DNA microarray analysis were performed to determine clonal distribution, diversity and gene content in regard to patients characteristics. In total, 107 isolates were recovered from 88 patients with clinical symptoms of invasive bacterial infection. The final set of 92 non-duplicate samples included 38 MRSA isolates. Additionally, a set of 54  S .  aureus  isolates collected during epidemiological screening was genotyped and analysed. There were 72 healthcare-associated (HCA) and 20 community-onset (CO) infection events caused by 33 and 5 MRSA isolates, respectively. The majority of isolates were affiliated with the major European clonal complexes CC5 (t003,  spa -CC 002), CC45 ( spa -CC 015), CC7 or CC15 (t084, t091,  spa -CC 084). Two epidemic clones (CC5-MRSA-II or CC45-MRSA-IV) dominated among MRSA isolates, while MSSA population contained 15 different CCs. The epidemiological screening isolates belonged to similar genetic lineages as those collected from invasive infection cases. The HCA infection events,  spa  types t003, t2642 or CC5 were significantly associated with infections occurring in neonates and children under 5 years of age. Moreover, carriage of several genetic markers, including  erm(A) ,  sea (N315) ,  egc- cluster,  chp  was significantly higher in isolates obtained from children in this age group. The  spa  types t091 and t008 were underrepresented among patients aged 5 years or younger, whereas  spa  type t008, CC8 and presence of  splE  was associated with infection in children aged 10 years or older. The HCA-MRSA strains were most frequently found in children under 5 years, although the majority of invasive infections was associated with MSSA strains. Moreover, an association between age group of children from the study population and a specific strain genotype ( spa  type, clonal complex or genetic content) was observed among the patients.

plos one

Clonal Structure and Characterization of Staphylococcus aureus Strains from Invasive Infections in Paediatric Patients from South Poland: Association between Age, spa Types, Clonal Complexes, and Genetic Markers