English (United Kingdom)

https://curated-unify.zendy.io/wp-json/zendy-region/v1/featured_content/oa?rat=en

https://curated-unify.zendy.io/wp-json/zendy-region/v1/highlighted_journal/

Global: Zendy Plus annual

Presents the access of premium content as premium feature

Premium Content

Presents the keyphrase highlighting as premium feature

Keyphrase Highlighting

Presents the summarisation as premium feature

Summarisation

Insights

Presents the pdf analysis as premium feature

PDF Analysis

Presents the zaia usage as premium feature

ZAIA

Global: Zendy Plus monthly

Global: Zendy Tools annual

Global: Zendy Tools monthly

Global: Zendy Free Plan

Pluripotency is maintained in mouse embryonic stem (ES) cells and is induced from somatic cells by the activation of appropriate transcriptional regulatory networks.  Krüppel-like factor  gene family members, such as  Klf2 ,  Klf4  and  Klf5 , have important roles in maintaining the undifferentiated state of mouse ES cells as well as in cellular reprogramming, yet it is not known whether other  Klf  family members exert self-renewal and reprogramming functions when overexpressed. In this study, we examined whether overexpression of any representative  Klf  family member, such as  Klf1–Klf10 , would be sufficient for the self-renewal of mouse ES cells. We found that only  Klf2 ,  Klf4 , and  Klf5  produced leukemia inhibitory factor (LIF)-independent self-renewal, although most KLF proteins, if not all, have the ability to occupy the regulatory regions of  Nanog , a critical Klf target gene. We also examined whether overexpression of any of  Klf1-Klf10  would be sufficient to convert epiblast stem cells into a naïve pluripotent state and found that  Klf5  had such reprogramming ability, in addition to  Klf2  and  Klf4 . We also delineated the functional domains of the  Klf2  protein for LIF-independent self-renewal and reprogramming. Interestingly, we found that both the N-terminal transcriptional activation and C-terminal zinc finger domains were indispensable for this activity. Taken together, our comprehensive analysis provides new insight into the contribution of  Klf  family members to mouse ES self-renewal and cellular reprogramming.

plos one

Comprehensive Identification of Krüppel-Like Factor Family Members Contributing to the Self-Renewal of Mouse Embryonic Stem Cells and Cellular Reprogramming