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The photosynthetic bradyrhizobia are able to use a Nod-factor independent process to induce nitrogen-fixing nodules on some semi-aquatic  Aeschynomene  species. These bacteria display a unique LPS  O -antigen composed of a new sugar, the bradyrhizose that is regarded as a key symbiotic factor due to its non-immunogenic character. In this study, to check this hypothesis, we isolated mutants affected in the  O -antigen synthesis by screening a transposon mutant library of the ORS285 strain for clones altered in colony morphology. Over the 10,000 mutants screened, five were selected and found to be mutated in two genes,  rfaL , encoding for a putative  O -antigen ligase and  gdh  encoding for a putative dTDP-glucose 4,6-dehydratase. Biochemical analysis confirmed that the LPS of these mutants completely lack the  O -antigen region. However, no effect of the mutations could be detected on the symbiotic properties of the mutants indicating that the  O -antigen region of photosynthetic  Bradyrhizobium  strains is not required for the establishment of symbiosis with  Aeschynomene .

The LPS O-Antigen in Photosynthetic Bradyrhizobium Strains Is Dispensable for the Establishment of a Successful Symbiosis with Aeschynomene Legumes