English (United Kingdom)

https://curated-unify.zendy.io/wp-json/zendy-region/v1/featured_content/oa?rat=en

https://curated-unify.zendy.io/wp-json/zendy-region/v1/highlighted_journal/

Global: Zendy Plus annual

Presents the access of premium content as premium feature

Premium Content

Presents the keyphrase highlighting as premium feature

Keyphrase Highlighting

Presents the summarisation as premium feature

Summarisation

Insights

Presents the pdf analysis as premium feature

PDF Analysis

Presents the zaia usage as premium feature

ZAIA

Global: Zendy Tools annual

Global: Zendy Free Plan

Global: Zendy Tools monthly

Global: Zendy Plus monthly

Genetically relevant mouse models need to recapitulate the hallmarks of human disease by permitting spatiotemporal gene targeting. This is especially important for replicating the biology of complex diseases like cancer, where genetic events occur in a sporadic fashion within developed somatic tissues. Though a number of renal tubule targeting mouse lines have been developed their utility for the study of renal disease is limited by lack of inducibility and specificity. In this study we describe the generation and characterisation of two novel mouse lines directing CreER T2  expression to renal tubular epithelia. The  Pax8 -CreER T2  transgenic line uses the mouse  Pax8  promoter to direct expression of CreER T2  to all renal tubular compartments (proximal and distal tubules as well as collecting ducts) whilst the  Slc22a6 -CreER T2  knock-in line utilises the endogenous mouse  Slc22a6  locus to specifically target the epithelium of proximal renal tubules. Both lines show high organ and tissue specificity with no extrarenal activity detected. To establish the utility of these lines for the study of renal cancer biology,  Pax8 -CreER T2  and  Slc22a6 -CreER T2  mice were crossed to conditional  Vhl  knockout mice to induce long-term renal tubule specific  Vhl  deletion. These models exhibited renal specific activation of the hypoxia inducible factor pathway (a VHL target). Our results establish  Pax8 -CreER T2  and  Slc22a6 -CreER T2  mice as valuable tools for the investigation and modelling of complex renal biology and disease.

plos one

Generation and Characterisation of a Pax8-CreERT2 Transgenic Line and a Slc22a6-CreERT2 Knock-In Line for Inducible and Specific Genetic Manipulation of Renal Tubular Epithelial Cells