English (United Kingdom)

https://curated-unify.zendy.io/wp-json/zendy-region/v1/featured_content/oa?rat=en

https://curated-unify.zendy.io/wp-json/zendy-region/v1/highlighted_journal/

Global: Zendy Plus annual

Presents the access of premium content as premium feature

Premium Content

Presents the keyphrase highlighting as premium feature

Keyphrase Highlighting

Presents the summarisation as premium feature

Summarisation

Insights

Presents the pdf analysis as premium feature

PDF Analysis

Presents the zaia usage as premium feature

ZAIA

Global: Zendy Tools annual

Global: Zendy Free Plan

Global: Zendy Tools monthly

Global: Zendy Plus monthly

Crocodilians are evolutionarily distinct reptiles that are distantly related to lizards and are thought to be the closest relatives of birds. Compared with birds and mammals, few studies have investigated the Ig light chain of crocodilians. Here, employing an  Alligator sinensis  genomic bacterial artificial chromosome (BAC) library and available genome data, we characterized the genomic organization of the  Alligator sinensis  IgL gene loci. The  Alligator sinensis  has two IgL isotypes, λ and κ, the same as  Anolis carolinensis . The Igλ locus contains 6 C λ  genes, each preceded by a J λ  gene, and 86 potentially functional V λ  genes upstream of (J λ -C λ ) n . The Igκ locus contains a single C κ  gene, 6 J κ s and 62 functional V κ s. All V L  genes are classified into a total of 31 families: 19 V λ  families and 12 V κ  families. Based on an analysis of the chromosomal location of the light chain genes among mammals, birds, lizards and frogs, the data further confirm that there are two IgL isotypes in the  Alligator sinensis : Igλ and Igκ. By analyzing the cloned Igλ/κ cDNA, we identified a biased usage pattern of V families in the expressed V λ  and V κ . An analysis of the junctions of the recombined VJ revealed the presence of N and P nucleotides in both expressed λ and κ sequences. Phylogenetic analysis of the V genes revealed V families shared by mammals, birds, reptiles and  Xenopus , suggesting that these conserved V families are orthologous and have been retained during the evolution of IgL. Our data suggest that the  Alligator sinensis  IgL gene repertoire is highly diverse and complex and provide insight into immunoglobulin gene evolution in vertebrates.

plos one

A Comprehensive Analysis of the Phylogeny, Genomic Organization and Expression of Immunoglobulin Light Chain Genes in Alligator sinensis, an Endangered Reptile Species