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2-Butanone is an important commodity chemical of wide application in different areas. In this study,  Klebsiella pneumoniae  was engineered to directly produce 2-butanone from glucose by extending its native 2, 3-butanediol synthesis pathway. To identify the potential enzyme for the efficient conversion of 2, 3-butanediol to 2-butanone, we screened different glycerol dehydratases and diol dehydratases. By introducing the diol dehydratase from  Lactobacillus brevis  and deleting the  ldhA  gene encoding lactate dehydrogenase, the engineered  K .  pneumoniae  was able to accumulate 246 mg/L of 2-butanone in shake flask. With further optimization of culture condition, the titer of 2-butanone was increased to 450 mg/L. This study lays the basis for developing an efficient biological process for 2-butanone production.

Metabolic Engineering of Klebsiella pneumoniae for the Production of 2-Butanone from Glucose