English (United Kingdom)

https://curated-unify.zendy.io/wp-json/zendy-region/v1/featured_content/oa?rat=en

https://curated-unify.zendy.io/wp-json/zendy-region/v1/highlighted_journal/

Global: Zendy Tools annual

Presents the keyphrase highlighting as premium feature

Keyphrase Highlighting

Presents the summarisation as premium feature

Summarisation

Insights

Presents the pdf analysis as premium feature

PDF Analysis

Presents the zaia usage as premium feature

ZAIA

Global: Zendy Tools monthly

Global: Zendy Plus monthly

Presents the access of premium content as premium feature

Premium Content

Global: Zendy Plus annual

Global: Zendy Free Plan

Bordetella bronchiseptica  causes infections of the respiratory tract in swine and other mammals and is a precursor for secondary infections with  Pasteurella multocida . Treatment of  B .  bronchiseptica  infections is conducted primarily with antimicrobial agents. Therefore it is essential to get an overview of the susceptibility status of these bacteria. The aim of this study was to comparatively analyse broth microdilution susceptibility testing according to CLSI recommendations with an incubation time of 16 to 20 hours and a longer incubation time of 24 hours, as recently proposed to obtain more homogenous MICs. Susceptibility testing against a panel of 22 antimicrobial agents and two fixed combinations was performed with 107 porcine isolates from different farms and regions in Germany and 43 isolates obtained from companion animals in Germany and other European countries. Isolates with increased MICs were investigated by PCR assays for the presence of resistance genes. For ampicillin, all 107 porcine isolates were classified as resistant, whereas only a single isolate was resistant to florfenicol. All isolates obtained from companion animals showed elevated MICs for β-lactam antibiotics and demonstrated an overall low susceptibility to cephalosporines. Extension of the incubation time resulted in 1–2 dilution steps higher MIC 50  values of porcine isolates for seven antimicrobial agents tested, while isolates from companion animals exhibited twofold higher MIC 50/90  values only for tetracycline and cefotaxime. For three antimicrobial agents, lower MIC 50  and MIC 90  values were detected for both, porcine and companion animal isolates. Among the 150 isolates tested, the resistance genes  bla   BOR-1  (n = 147),  bla   OXA-2 , (n = 4),  strA  and  strB  (n = 17),  sul1  (n = 10),  sul2  (n = 73),  dfrA7  (n = 3) and  tet (A) (n = 8) were detected and a plasmid localisation was identified for several of the resistance genes.

Antimicrobial Susceptibility of Bordetella bronchiseptica Isolates from Swine and Companion Animals and Detection of Resistance Genes