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Astragalus  species are medicinal plants that are used in the world for years. Some  Astragalus  species are known for selenium accumulation and tolerance and one of them is  Astragalus chrysochlorus , a secondary selenium accumulator. In this study, we employed Illumina deep sequencing technology for the first time to  de novo  assemble  A .  chrysochlorus  transcriptome and identify the differentially expressed genes after selenate treatment. Totally, 59,656 unigenes were annotated with different databases and 53,960 unigenes were detected in NR database. Transcriptome in  A .  chrysochlorus  is closer to  Glycine max  than other plant species with 43,1 percentage of similarity. Annotated unigenes were also used for gene ontology enrichment and pathway enrichment analysis. The most significant genes and pathways were ABC transporters, plant pathogen interaction, biosynthesis of secondary metabolites and carbohydrate metabolism. Our results will help to enlighten the selenium accumulation and tolerance mechanisms, respectively in plants.

plos one

De Novo Transcriptome Assembly and Comparative Analysis Elucidate Complicated Mechanism Regulating Astragalus chrysochlorus Response to Selenium Stimuli