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A functional rs4245739 A>C single nucleotide polymorphism (SNP) locating in the  MDM4 3’-untranslated (3’-UTR) region creates a miR-191-5p or miR-887-3p targeting sites. This change results in decreased expression of oncogene  MDM4 . Therefore, we examined the association between this SNP and small cell lung cancer (SCLC) risk as well as its regulatory function in SCLC cells. Genotypes were determined in two independent case-control sets consisted of 520SCLC cases and 1040 controls from two regions of China. Odds ratios (ORs) and 95% confidence intervals (CIs) were estimated by logistic regression. The impact of the rs4245739 SNP on miR-191-5p/miR-887-3p mediated  MDM4  expression regulation was investigated using luciferase reporter gene assays. We found that the  MDM4  rs4245739AC and CC genotypes were significantly associated with decreased SCLC susceptibility compared with the AA genotype in both case-control sets (Shandong set: OR = 0.53, 95% CI = 0.32–0.89,  P  = 0.014; Jiangsu set: OR = 0.47, 95% CI = 0.26–0.879,  P  = 0.017). Stratified analyses indicated that there was a significantly multiplicative interaction between rs4245739 and smoking ( P   interactioin  = 0.048). After co-tranfection of miRNAs and different allelic- MDM4  reporter constructs into SCLC cells, we found that the both miR-191-5p and miR-887-3p can lead to significantly decreased  MDM4  expression activities in the construct with C-allelic 3’-UTR but not A-allelic 3’-UTR, suggesting a consistent genotype-phenotype correlation. Our data illuminate that the  MDM4 rs4245739SNP contributes to SCLC risk and support the notion that gene 3’-UTR genetic variants, impacting miRNA-binding, might modify SCLC susceptibility.

A Regulatory MDM4 Genetic Variant Locating in the Binding Sequence of Multiple MicroRNAs Contributes to Susceptibility of Small Cell Lung Cancer