English (United Kingdom)

https://curated-unify.zendy.io/wp-json/zendy-region/v1/featured_content/oa?rat=en

https://curated-unify.zendy.io/wp-json/zendy-region/v1/highlighted_journal/

Global: Zendy Plus annual

Presents the access of premium content as premium feature

Premium Content

Presents the keyphrase highlighting as premium feature

Keyphrase Highlighting

Presents the summarisation as premium feature

Summarisation

Insights

Presents the pdf analysis as premium feature

PDF Analysis

Presents the zaia usage as premium feature

ZAIA

Global: Zendy Tools annual

Global: Zendy Free Plan

Global: Zendy Tools monthly

Global: Zendy Plus monthly

Some  Pseudomonas aeruginosa  strains including Australian Epidemic Strain-1 (AES-1 or AUS-01) cause persistent chronic infection in cystic fibrosis (CF) patients, with greater morbidity and mortality. Factors conferring persistence are largely unknown. Previously we analysed the transcriptomes of AES-1 grown in Luria broth, nematode growth medium for  Caenorhabditis elegan s assay (both aerobic) and artificial sputum medium (mainly hypoxic). Transcriptional comparisons included chronic AES-1 strains against PAO1 and acute AES-1 (AES-1R) against its chronic isogen (AES-1M), isolated 10.5 years apart from a CF patient and not eradicated in the meantime. Prominent amongst genes downregulated in AES-1M in all comparisons was homogentisate-1-2-dioxygenase ( hmgA ); an oxygen-dependent gene known to be mutationally deactivated in many chronic infection strains of  P .  aeruginosa . To investigate if  hmgA  downregulation and deactivation gave similar virulence persistence profiles, a  hmgA  mutant made in UCBPP-PA14 utilising RedS-recombinase and AES-1M were assessed in the  C .  elegan s virulence assay, and the C57BL/6 mouse for pulmonary colonisation and TNF-α response. In  C .  elegans ,  hmgA  deactivation resulted in significantly increased PA14 virulence while  hmgA  downregulation reduced AES-1M virulence. AES-1M was significantly more persistent in mouse lung and showed a significant increase in TNF-α (p<0.0001), sustained even with no detectable bacteria. PA14Δ hmgA  did not show increased TNF-α. This study suggests that  hmgA  may have a role in  P .  aeruginosa  persistence in chronic infection and the results provide a starting point for clarifying the role of  hmgA  in chronic AES-1.

Homogentisate 1-2-Dioxygenase Downregulation in the Chronic Persistence of Pseudomonas aeruginosa Australian Epidemic Strain-1 in the CF Lung