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Bacterial two-component regulatory systems (TCSs) facilitate changes in gene expression in response to environmental stimuli. TCS BaeR regulons influence tigecycline susceptibility in  Acinetobacter baumannii  through positively regulating the pump genes  adeA  and  adeB . In this study, we demonstrate that an additional two transport systems, AdeIJK and MacAB-TolC, are also regulated by BaeSR. In the wild type and clinical tigecycline-resistant  A .  baumannii  strains, gene expression of AdeIJK and MacAB-TolC increased after tigecycline induction, implicating their importance to tigecycline resistance in addition to AdeABC. Phenotypic microarray results showed that  A .  baumannii  is vulnerable to certain chemicals, especially tannic acid, after deleting  baeR , which was confirmed using the spot assay. The wild-type strain of  A .  baumannii  also exhibited 1.6-fold and 4.4-fold increase in gene expression of  adeJ  and  macB  in the medium with 100 μg/mL tannic acid, but the increase was fully inhibited by  baeR  deletion. An electrophoretic motility shift assay based on an interaction between His-BaeR and the  adeA ,  adeI  and  macA  promoter regions did not demonstrate direct binding. In conclusion,  A .  baumannii  can use the TCS BaeSR in disposing chemicals, such as tannic acid and tigecycline, through regulating the efflux pumps.

The Role of the Two-Component System BaeSR in Disposing Chemicals through Regulating Transporter Systems in Acinetobacter baumannii