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The GapC of  Streptococcus dysgalactiae  ( S .  dysgalactiae ) is a highly conserved surface protein that can induce protective humoral immune response in animals. However, B-cell epitopes on the  S .  dysgalactiae  GapC have not been well identified. In this study, a monoclonal antibody (mAb5B7) against the GapC 1-150  protein was prepared. After passive transfer, mAb5B7 could partially protect mice against  S .  dysgalactiae  infection. Eleven positive phage clones recognized by mAb5B7 were identified by screening phage-displayed random 12-peptide library, most of which matched the consensus motif DTTQGRFD. The motif sequence exactly matches amino acids 48-55 of the  S .  dysgalactiae  GapC protein. In addition, the motif  48 DTTQGRFD 55  shows high homology among various  streptococcus  species. Site-directed mutagenic analysis further confirmed that residues D48, T50, Q51, G52 and F54 formed the core motif of  48 DTTQGRFD 55 . This motif was the minimal determinant of the B-cell epitope recognized by the mAb5B7. As expected, epitope-peptide evoked protective immune response against  S .  dysgalactiae  infection in immunized mice. Taken together, this identified conserved B-cell epitope within  S .  dysgalactiae  GapC could provide very valuable insights for vaccine design against  S .  dysgalactiae  infection.

Identification of a Conserved Linear B-Cell Epitope of Streptococcus dysgalactiae GapC Protein by Screening Phage-Displayed Random Peptide Library