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The type 1 ryanodine receptor (RyR1) is a Ca 2+  release channel in the sarcoplasmic reticulum of skeletal muscle and is mutated in several diseases, including malignant hyperthermia (MH) and central core disease (CCD). Most MH and CCD mutations cause accelerated Ca 2+  release, resulting in abnormal Ca 2+  homeostasis in skeletal muscle. However, how specific mutations affect the channel to produce different phenotypes is not well understood. In this study, we have investigated 11 mutations at 7 different positions in the amino (N)-terminal region of RyR1 (9 MH and 2 MH/CCD mutations) using a heterologous expression system in HEK293 cells. In live-cell Ca 2+  imaging at room temperature (~25 °C), cells expressing mutant channels exhibited alterations in Ca 2+  homeostasis, i.e., an enhanced sensitivity to caffeine, a depletion of Ca 2+  in the ER and an increase in resting cytoplasmic Ca 2+ . RyR1 channel activity was quantitatively evaluated by [ 3 H]ryanodine binding and three parameters (sensitivity to activating Ca 2+ , sensitivity to inactivating Ca 2+  and attainable maximum activity, i.e., gain) were obtained by fitting analysis. The mutations increased the gain and the sensitivity to activating Ca 2+  in a site-specific manner. The gain was consistently higher in both MH and MH/CCD mutations. Sensitivity to activating Ca 2+  was markedly enhanced in MH/CCD mutations. The channel activity estimated from the three parameters provides a reasonable explanation to the pathological phenotype assessed by Ca 2+  homeostasis. These properties were also observed at higher temperatures (~37 °C). Our data suggest that divergent activity profiles may cause varied disease phenotypes by specific mutations. This approach should be useful for diagnosis and treatment of diseases with mutations in RyR1.

plos one

Divergent Activity Profiles of Type 1 Ryanodine Receptor Channels Carrying Malignant Hyperthermia and Central Core Disease Mutations in the Amino-Terminal Region