English (United Kingdom)

https://curated-unify.zendy.io/wp-json/zendy-region/v1/featured_content/oa?rat=en

https://curated-unify.zendy.io/wp-json/zendy-region/v1/highlighted_journal/

Global: Zendy Plus annual

Presents the access of premium content as premium feature

Premium Content

Presents the keyphrase highlighting as premium feature

Keyphrase Highlighting

Presents the summarisation as premium feature

Summarisation

Insights

Presents the pdf analysis as premium feature

PDF Analysis

Presents the zaia usage as premium feature

ZAIA

Global: Zendy Tools annual

Global: Zendy Free Plan

Global: Zendy Tools monthly

Global: Zendy Plus monthly

Powdery mildew (PM), caused by  Blumeria graminis  f. sp.  tritici , is one of the important wheat diseases, worldwide. Two PM resistance genes, designated as  PmTb7A . 1  and  PmTb7A . 2 , were identified in  T .  boeoticum  acc. pau5088 and mapped on chromosome 7AL approximately 48cM apart. Two resistance gene analogue (RGA)-STS markers  Ta7AL-4556232  and  7AL-4426363  were identified to be linked to the  PmTb7A . 1  and  PmTb7A . 2 , at a distance of 0.6cM and 6.0cM, respectively. In the present study, following marker assisted selection (MAS), the two genes were transferred to  T .  aestivum  using  T .  durum  as bridging species. As many as 12,317 florets of F 1  of the cross  T .  durum  / T .  boeoticum  were pollinated with  T .  aestivum  lines PBW343-IL and PBW621 to produce 61 and 65 seeds, respectively, of three-way F 1 . The resulting F 1 s of the cross  T .  durum/T .  boeoticum//T .  aestivum  were screened with marker flanking both the PM resistance genes  PmTb7A . 1  and  PmTb7A . 2  (foreground selection) and the selected plants were backcrossed to generate BC 1 F 1 . Marker assisted selection was carried both in BC 1 F 1  and the BC 2 F 1  generations. Introgression of alien chromatin in BC 2 F 1  plants varied from 15.4 - 62.9 percent. Out of more than 110 BC 2 F 1  plants showing introgression for markers linked to the two PM resistance genes, 40 agronomically desirable plants were selected for background selection for the carrier chromosome to identify the plants with minimum of the alien introgression. Cytological analysis showed that most plants have chromosome number ranging from 40-42. The BC 2 F 2  plants homozygous for the two genes have been identified. These will be crossed to generate lines combining both the PM resistance genes but with minimal of the alien introgression. The PM resistance gene  PmTb7A . 1  maps in a region very close to  Sr22 , a stem rust resistance gene effective against the race Ug99. Analysis of selected plants with markers linked to  Sr22  showed introgression of  Sr22  from  T .  boeoticum  in several BC 2 F 1  plants. Thus, in addition to PM resistance, these progeny might also carry resistance to stem rust race Ug99.

Marker Assisted Transfer of Two Powdery Mildew Resistance Genes PmTb7A.1 and PmTb7A.2 from Triticum boeoticum (Boiss.) to Triticum aestivum (L.)