English (United Kingdom)

https://curated-unify.zendy.io/wp-json/zendy-region/v1/featured_content/oa?rat=en

https://curated-unify.zendy.io/wp-json/zendy-region/v1/highlighted_journal/

Global: Zendy Plus annual

Presents the access of premium content as premium feature

Premium Content

Presents the keyphrase highlighting as premium feature

Keyphrase Highlighting

Presents the summarisation as premium feature

Summarisation

Insights

Presents the pdf analysis as premium feature

PDF Analysis

Presents the zaia usage as premium feature

ZAIA

Global: Zendy Tools annual

Global: Zendy Free Plan

Global: Zendy Tools monthly

Global: Zendy Plus monthly

Malachite green (MG) was decolorized by laccase (LacA) of white-rot fungus  Cerrena  sp. with strong decolorizing ability. Decolorization conditions were optimized with response surface methodology. A highly significant quadratic model was developed to investigate MG decolorization with LacA, and the maximum MG decolorization ratio of 91.6% was predicted under the conditions of 2.8 U mL -1  LacA, 109.9 mg L -1  MG and decolorization for 172.4 min. Kinetic studies revealed the  K m and  k cat values of LacA toward MG were 781.9 mM and 9.5 s -1 , respectively. UV–visible spectra confirmed degradation of MG, and the degradation mechanism was explored with liquid chromatography–mass spectrometry (LC-MS) analysis. Based on the LC-MS spectra of degradation products, LacA catalyzed MG degradation via two simultaneous pathways. In addition, the phytotoxicity of MG, in terms of inhibition on seed germination and seedling root elongation of  Nicotiana tabacum  and  Lactuca sativa , was reduced after laccase treatment. These results suggest that laccase of  Cerrena  was effective in decolorizing MG and promising in bioremediation of wastewater in food and aquaculture industries.

Laccase-Catalyzed Decolorization of Malachite Green: Performance Optimization and Degradation Mechanism