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An activation-tagging methodology was applied to dedifferentiated calli of  Arabidopsis  to identify new genes involved in salt tolerance. This identified  salt tolerant callus 8  ( stc8 ) as a gene encoding the basic helix-loop-helix transcription factor bHLH106.  bHLH106 -knockout (KO) lines were more sensitive to NaCl, KCl, LiCl, ABA, and low temperatures than the wild-type. Back-transformation of the KO line rescued its phenotype, and over-expression (OX) of  bHLH106  in differentiated plants exhibited tolerance to NaCl. Green fluorescent protein (GFP) fused with bHLH106 revealed that it was localized to the nucleus. Prepared bHLH106 protein was subjected to electrophoresis mobility shift assays against E-box sequences ( 5'-CANNTG-3' ). The G-box sequence  5'-CACGTG-3'  had the strongest interaction with bHLH106.  bHLH106 -OX lines were transcriptomically analyzed, and resultant up- and down-regulated genes selected on the criterion of presence of a G-box sequence. There were 198 genes positively regulated by bHLH106 and 36 genes negatively regulated; these genes possessed one or more G-box sequences in their promoter regions. Many of these genes are known to be involved in abiotic stress response. It is concluded that bHLH106 locates at a branching point in the abiotic stress response network by interacting directly to the G-box in genes conferring salt tolerance on plants.

bHLH106 Integrates Functions of Multiple Genes through Their G-Box to Confer Salt Tolerance on Arabidopsis