English (United Kingdom)

https://curated-unify.zendy.io/wp-json/zendy-region/v1/featured_content/oa?rat=en

https://curated-unify.zendy.io/wp-json/zendy-region/v1/highlighted_journal/

Global: Zendy Plus annual

Presents the access of premium content as premium feature

Premium Content

Presents the keyphrase highlighting as premium feature

Keyphrase Highlighting

Presents the summarisation as premium feature

Summarisation

Insights

Presents the pdf analysis as premium feature

PDF Analysis

Presents the zaia usage as premium feature

ZAIA

Global: Zendy Tools annual

Global: Zendy Free Plan

Global: Zendy Tools monthly

Global: Zendy Plus monthly

Candida albicans  is the most frequently isolated human fungal pathogen and can cause a range of mucosal and systemic infections in immunocompromised individuals. Morphogenesis, the ability to undergo a reversible transition from budding yeast to elongated filaments, is an essential virulence trait. The yeast-to-filament transition is associated with expression of genes specifically important for filamentation as well as other virulence-related processes, and is controlled, in part, by the key transcriptional regulators Nrg1 and Ume6. Both of these regulators are themselves controlled at the transcriptional level by filament-inducing environmental cues, although little is known about how this process occurs. In order to address this question and determine whether environmental signals regulate transcription of  UME6  and  NRG1  via distinct and/or common promoter elements, we performed promoter deletion analyses. Strains bearing promoter deletion constructs were induced to form filaments in YEPD plus 10% serum at 37°C, Spider medium (nitrogen and carbon starvation) and/or Lee’s medium pH 6.8 (neutral pH) and reporter gene expression was measured. In the  NRG1  promoter we identified several distinct condition-specific response elements for YEPD plus 10% serum at 37°C and Spider medium. In the  UME6  promoter we also identified response elements for YEPD plus 10% serum at 37°C. While a few of these elements are distinct, others overlap with those which respond to Lee’s pH 6.8 medium. Consistent with  UME6  possessing a very long 5’ UTR, many response elements in the  UME6  promoter are located significantly upstream from the coding sequence. Our data indicate that certain distinct condition-specific elements can control expression of  C .  albicans UME6  and  NRG1  in response to key filament-inducing environmental cues. Because  C .  albicans  encounters a variety of host microenvironments during infection, our results suggest that  UME6  and  NRG1  expression can be differentially modulated by multiple signaling pathways to control filamentation and virulence  in vivo .

plos one

Filament Condition-Specific Response Elements Control the Expression of NRG1 and UME6, Key Transcriptional Regulators of Morphology and Virulence in Candida albicans