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Plant hormone brassinosteroids (BRs) are a group of polyhydroxylated steroids that play critical roles in regulating broad aspects of plant growth and development. The structural diversity of BRs is generated by the action of several groups of P450s. Brassinazole is a specific inhibitor of C-22 hydroxylase (CYP90B1) in BR biosynthesis, and the application use of brassinazole has emerged as an effective way of complementing BR-deficient mutants to elucidate the functions of BRs. In this article, we report a new triazole-type BR biosynthesis inhibitor,  YCZ-18 . Quantitative analysis the endogenous levels of BRs in  Arabidopsis  indicated that  YCZ-18  significantly decreased the BR contents in plant tissues. Assessment of the binding affinity of  YCZ-18 to purified recombinant CYP90D1 indicated that  YCZ-18  induced a typical type II binding spectrum with a K d  value of approximately 0.79 μM. Analysis of the mechanisms underlying the dwarf phenotype associated with  YCZ-18  treatment of  Arabidopsis  indicated that the chemically induced dwarf phenotype was caused by a failure of cell elongation. Moreover, dissecting the effect of  YCZ-18  on the induction or down regulation of genes responsive to BRs indicated that  YCZ-18  regulated the expression of genes responsible for BRs deficiency in  Arabidopsis . These findings indicate that  YCZ-18  is a potent BR biosynthesis inhibitor and has a new target site, C23-hydroxylation in BR biosynthesis. Application of  YCZ-18  will be a good starting point for further elucidation of the detailed mechanism of BR biosynthesis and its regulation.

YCZ-18 Is a New Brassinosteroid Biosynthesis Inhibitor