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REV3L, the catalytic subunit of DNA Polymerase ζ (Polζ), plays a significant role in the DNA damage tolerance mechanism of translesion synthesis (TLS). The role of  REV3L  in chemosensitivity of cervical cancer needs exploration. In the present study, we evaluated the expression of the Polζ protein in paraffin-embedded tissues using immunohistochemistry and found that the expression of Polζ in cervical cancer tissues was higher than that in normal tissues. We then established some cervical cancer cell lines with  REV3L  suppression or overexpression. Depletion of  REV3L  suppresses cell proliferation and colony formation of cervical cancer cells through G 1  arrest, and  REV3L  promotes cell proliferation and colony formation of cervical cancer cells by promoting G 1  phase to S phase transition. The suppression of  REV3L  expression enhanced the sensitivity of cervical cancer cells to cisplatin, and the overexpression of  REV3L  conferred resistance to cisplatin as evidenced by the alteration of apoptosis rates, and significantly expression level changes of anti-apoptotic proteins B-cell lymphoma 2 (Bcl-2), myeloid cell leukemia sequence 1 (Mcl-1) and B-cell lymphoma-extra large (Bcl-xl) and proapoptotic Bcl-2-associated x protein (Bax). Our data suggest that  REV3L  plays an important role in regulating cervical cancer cellular response to cisplatin, and thus targeting  REV3L  may be a promising way to alter chemosensitivity in cervical cancer patients.

REV3L, a Promising Target in Regulating the Chemosensitivity of Cervical Cancer Cells