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In order to investigate the factors controlling the bacterial community composition (BCC) in reservoirs, we sampled three freshwater reservoirs with contrasted physical and chemical characteristics and trophic status. The BCC was analysed by 16S rRNA gene amplicon 454 pyrosequencing. In parallel, a complete dataset of environmental parameters and phytoplankton community composition was also collected. BCC in the analysed reservoirs resembled that of epilimnetic waters of natural freshwater lakes with presence of  Actinobacteria ,  Alpha- and Betaproteobacteria ,  Cytophaga–Flavobacteria–Bacteroidetes  (CFB) and  Verrucomicrobia  groups. Our results evidenced that the retrieved BCC in the analysed reservoirs was strongly influenced by pH, alkalinity and organic carbon content, whereas comparatively little change was observed among layers in stratified conditions.

Bacterial Community Composition in Three Freshwater Reservoirs of Different Alkalinity and Trophic Status