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MgtC is a virulence factor involved in intramacrophage growth that has been reported in several intracellular pathogens, including  Mycobacterium tuberculosis  and  Salmonella enterica  serovar Typhimurium. MgtC participates also in adaptation to Mg 2+  deprivation. Herein, we have constructed a  mgtC  mutant in  Mycobacterium marinum  to further investigate the role of MgtC in mycobacteria. We show that the  M. marinum mgtC  gene ( Mma mgtC ) is strongly induced upon Mg 2+  deprivation and is required for optimal growth in Mg 2+ -deprived medium. The behaviour of the  Mma mgtC  mutant has been investigated in the  Danio rerio  infection model using a transgenic reporter zebrafish line that specifically labels neutrophils. Although the  mgtC  mutant is not attenuated in the zebrafish embryo model based on survival curves, our results indicate that phagocytosis by neutrophils is enhanced with the  mgtC  mutant compared to the wild-type strain following subcutaneous injection. Increased phagocytosis of the mutant strain is also observed  ex vivo  with the murine J774 macrophage cell line. On the other hand, no difference was found between the  mgtC  mutant and the wild-type strain in bacterial adhesion to macrophages and in the internalization into epithelial cells. Unlike the role reported for MgtC in other intracellular pathogens,  Mma  MgtC does not contribute significantly to intramacrophage replication. Taken together, these results indicate an unanticipated function of  Mma  MgtC at early step of infection within phagocytic cells. Hence, our results indicate that although the MgtC function is conserved among pathogens regarding adaptation to Mg 2+  deprivation, its role towards phagocytic cells can differ, possibly in relation with the specific pathogen's lifestyles.

Mycobacterium marinum MgtC Plays a Role in Phagocytosis but is Dispensable for Intracellular Multiplication