English (United Kingdom)

https://curated-unify.zendy.io/wp-json/zendy-region/v1/featured_content/oa?rat=en

https://curated-unify.zendy.io/wp-json/zendy-region/v1/highlighted_journal/

Global: Zendy Plus annual

Presents the access of premium content as premium feature

Premium Content

Presents the keyphrase highlighting as premium feature

Keyphrase Highlighting

Presents the summarisation as premium feature

Summarisation

Insights

Presents the pdf analysis as premium feature

PDF Analysis

Presents the zaia usage as premium feature

ZAIA

Global: Zendy Plus monthly

Global: Zendy Tools annual

Global: Zendy Tools monthly

Global: Zendy Free Plan

Background  Advancements have been made in the genetic manipulation of apicomplexan parasites. Both the  in vitro  transient and  in vivo  stable transfection of  Eimeria tenella  have been developed successfully. Herein, we report the transient and stable transfection of  Eimeria mitis .    Methods and Findings  Sporozoites of  E. mitis  transfected with enhanced yellow fluorescent protein (EYFP) expression plasmid were inoculated into chickens via the cloacal route. The recovered fluorescent oocysts were sorted by fluorescence activated cell sorting (FACS) and then passaged 6 generations successively in chickens. The resulting population was analyzed by genome walking and Western blot. The endogenous development of the transgenic  E. mitis  was observed and its reproduction potential was tested. The stable transfection of  E. mitis  was developed. Genome walking confirmed the random integration of plasmid DNA into the genome; while Western blot analysis demonstrated the expression of foreign proteins. Constitutive expression of EYFP was observed in all stages of merogony, gametogony and sporogony. The peak of the transgenic oocyst output was delayed by 24 h and the total oocyst reproduction was reduced by 7-fold when compared to the parental strain.    Conclusion  Stable transfection of  E. mitis  was successfully developed. The expression of foreign antigens in the transgenic parasites will facilitate the development of transgenic  E. mitis  as a vaccine vector.

Transfection of Eimeria mitis with Yellow Fluorescent Protein as Reporter and the Endogenous Development of the Transgenic Parasite