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Copyright: 2014 The PLOS ONE Staff. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. Figure 3. Hypoxia-driven inhibition of expression of hallmarks of senescence is Hif-1a dependent. shRNA_HIF-1a and shRNA_scr (a scrambled shRNA sequence encoding plasmid used as negative control) expressing IMR-90 and BJ cells were infected with H-RasV12 and selected for puromycin for 3 days. Three days post exposure to hypoxia cells were analyzed for A. the expression of HIF-1a by western-blotting, b-actin was used as loading control; B. for mRNA level by Quantitative RT-PCR; C. the expression of senescence regulators p53, p21CIP1, p16INK4a and MIF by westernblotting. b-actin was used as loading control. Statistically significant differences between mRNA levels of HIF-1a in Ras + shNC vs. Ras+ shHIF-1a expressing cells in hypoxia are indicated *, p,0.01. Shown are means 6 SD of 3 independent experiments in triplets. doi:10.1371/journal.pone.0101064.g003

Correction: The Role of Hypoxia Inducible Factor-1 Alpha in Bypassing Oncogene-Induced Senescence