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Clathrin heavy chain has been shown to be important for viability, embryogenesis, and RNA interference (RNAi) in arthropods such as  Drosophila melanogaster . However, the functional roles of clathrin heavy chain in chelicerate arthropods, such as the predatory mite  Metaseiulus occidentalis,  remain unknown. We previously showed that dsRNA ingestion, followed by feeding on spider mites, induced systemic and robust RNAi in  M. occidentalis  females. In the current study, we performed a loss-of-function analysis of the  clathrin heavy chain  gene in  M. occidentalis  using RNAi. We showed that ingestion of  clathrin heavy chain  dsRNA by  M. occidentalis  females resulted in gene knockdown and reduced longevity. In addition,  clathrin heavy chain  dsRNA treatment almost completely abolished oviposition by  M. occidentalis  females and the few eggs produced did not hatch. Finally, we demonstrated that  clathrin heavy chain  gene knockdown in  M. occidentalis  females significantly reduced a subsequent RNAi response induced by ingestion of  cathepsin L  dsRNA. The last finding suggests that clathrin heavy chain may be involved in systemic RNAi responses mediated by orally delivered dsRNAs in  M. occidentalis .

plos one

Clathrin Heavy Chain Is Important for Viability, Oviposition, Embryogenesis and, Possibly, Systemic RNAi Response in the Predatory Mite Metaseiulus occidentalis