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Background  Orthopteran migratory locust,  Locusta migratoria , and lepidopteran Asian corn borer,  Ostrinia furnacalis , are two types of insects undergoing incomplete and complete metamorphosis, respectively. Identification of candidate genes regulating wing development in these two insects would provide insights into the further study about the molecular mechanisms controlling metamorphosis development. We have sequenced the transcriptome of  O. furnacalis  larvae previously. Here we sequenced and characterized the transcriptome of  L. migratoria  wing discs with special emphasis on wing development-related signaling pathways.    Methodology/Principal Findings  Illumina Hiseq2000 was used to sequence 8.38 Gb of the transcriptome from dissected nymphal wing discs.  De novo  assembly generated 91,907 unigenes with mean length of 610 nt. All unigenes were searched against five databases including Nt, Nr, Swiss-Prot, COG, and KEGG for annotations using blastn or blastx algorithm with an cut-off E-value of 10 −5 . A total of 23,359 (25.4%) unigenes have homologs within at least one database. Based on sequence similarity to homologs known to regulate  Drosophila melanogaster  wing development, we identified 50 and 46 potential wing development-related unigenes from  L. migratoria  and  O. furnacalis  transcriptome, respectively. The identified unigenes encode putative orthologs for nearly all components of the Hedgehog (Hh), Decapentaplegic (Dpp), Notch (N), and Wingless (Wg) signaling pathways, which are essential for growth and pattern formation during wing development. We investigated the expression profiles of the component genes involved in these signaling pathways in forewings and hind wings of  L. migratoria  and  O. furnacalis . The results revealed the tested genes had different expression patterns in two insects.    Conclusions/Significance  This study provides the comprehensive sequence resource of the wing development-related signaling pathways of  L. migratoria . The obtained data gives an insight into better understanding the molecular mechanisms involved in the wing development in  L. migratoria  and  O. furnacalis , two insect species with different metamorphosis types.

De Novo Transcriptome Analysis of Wing Development-Related Signaling Pathways in Locusta migratoria Manilensis and Ostrinia furnacalis (Guenée)