English (United Kingdom)

https://curated-unify.zendy.io/wp-json/zendy-region/v1/featured_content/oa?rat=en

https://curated-unify.zendy.io/wp-json/zendy-region/v1/highlighted_journal/

Global: Zendy Plus annual

Presents the access of premium content as premium feature

Premium Content

Presents the keyphrase highlighting as premium feature

Keyphrase Highlighting

Presents the summarisation as premium feature

Summarisation

Insights

Presents the pdf analysis as premium feature

PDF Analysis

Presents the zaia usage as premium feature

ZAIA

Global: Zendy Tools annual

Global: Zendy Free Plan

Global: Zendy Tools monthly

Global: Zendy Plus monthly

Objectives  The  in   vivo  efficacy of a cefotaxime-ciprofloxacin combination against  Vibrio vulnificus  and the effects on  rtxA1  expression of commonly used antibiotics are unknown.    Methods    In vitro  time-kill studies were performed to evaluate synergism. Female BALB/c mice were injected subcutaneously with 1×10 7  or 1×10 8  cfu of  V. vulnificus . Antibiotic therapy was initiated at 2 h after inoculation in the following four therapy groups: cefotaxime; ciprofloxacin; cefotaxime-plus-ciprofloxacin; and cefotaxime-plus-minocycline. The cytotoxicity of  V. vulnificus  for HeLa cells was measured using the lactate dehydrogenase assay;  rtxA1  transcription was measured in a transcriptional reporter strain using a β-galactosidase assay.    Results    In vitro  time-kill assays exhibited synergism between cefotaxime and ciprofloxacin. In the animal experiments, the 96-h survival rate for the cefotaxime-plus-ciprofloxacin group (85%; 17/20) was significantly higher than that of the cefotaxime-plus-minocycline (35%; 7/20) and cefotaxime alone (0%; 0/20) groups ( P <0.05 for both). Bacterial counts in the liver and spleen were significantly lower in the cefotaxime-plus-ciprofloxacin group 24 and 48 h after treatment, relative to the other groups. At sub-inhibitory concentrations, ciprofloxacin inhibited more effectively  rtxA1  transcription and mammalian cell cytotoxicity than either minocycline or cefotaxime ( P <0.05 for both).    Conclusions  Ciprofloxacin is more effective at reducing  rtxA1  transcription and subsequent cytotoxicity than either minocycline or cefotaxime, and the combination of ciprofloxacin and cefotaxime was more effective in clearing  V. vulnificus in   vivo  than previously used regimens. These data suggest that the combination of ciprofloxacin and cefotaxime is an effective option for the treatment of  V. vulnificus  sepsis in humans.

In Vivo Efficacy of the Combination of Ciprofloxacin and Cefotaxime against Vibrio vulnificus Sepsis