English (United Kingdom)

https://curated-unify.zendy.io/wp-json/zendy-region/v1/featured_content/oa?rat=en

https://curated-unify.zendy.io/wp-json/zendy-region/v1/highlighted_journal/

Global: Zendy Plus annual

Presents the access of premium content as premium feature

Premium Content

Presents the keyphrase highlighting as premium feature

Keyphrase Highlighting

Presents the summarisation as premium feature

Summarisation

Insights

Presents the pdf analysis as premium feature

PDF Analysis

Presents the zaia usage as premium feature

ZAIA

Global: Zendy Tools annual

Global: Zendy Free Plan

Global: Zendy Tools monthly

Global: Zendy Plus monthly

Background  Cystic echinococcosis is highly prevalent in northwest China. A cost-effective, easy to operate diagnostic tool with high sensitivity and specificity would greatly facilitate the monitoring of  Echinococcus  infections in canine definitive hosts.    Methods  The primers used in the LAMP assay were based on the mitochondrial  nad 5 gene of  E. granulosus sensu stricto  ( E. granulosus s.s. , or  E.g.s.s. ) and were designed using Primer Explorer V4 software. The developed LAMP assay was compared with a conventional PCR method, copro-ELISA and microscopy, using the faeces of dogs experimentally infected with  E.g.s.s. , and field-collected faeces of domestic dogs including 190 from Qinghai province highly endemic for  E.g.s.s.  and 30 controls from an area in Gansu, where a domestic dog de-worming program was in operation.    Results  The positivity rates obtained for the field-collected faecal samples were 12.6%, 1.6% and 2.1% by the LAMP, PCR and copro-ELISA assays, respectively. All samples obtained from the control dogs were negative. Compared with the conventional PCR, the LAMP assay provided 88.8% specificity and 100% sensitivity. The higher sensitivity of the LAMP method was also shown by the fact that it could detect the presence of laboratory challenge dog infections of  E. granulsous s.s.  four days earlier than the PCR method. Three copro-samples shown positive by the commercial copro-ELISA were all negative by LAMP, PCR and microscopy, which suggests these samples may have originated from another infection rather than  E. granulsous s.s. , possibly  E. shiquicus  or  E. Canadensis,  which is also present in China.    Conclusions  We have developed a potentially useful surveillance tool for determining the prevalence of canine  E. granulosus s.s.  infections in the field. The LAMP assay may lead to a more cost-effective and practicable way of tracking  Echinococcus  infections in canids, especially when combined with the copro-ELISA.

plos one

A Comparison of Loop-Mediated Isothermal Amplification (LAMP) with Other Surveillance Tools for Echinococcus granulosus Diagnosis in Canine Definitive Hosts